Experimental Toxocara canis infection in chickens

The objective of this study was to determine the development phases Toxocara canis eggs outside host and migration larvae in internal organs chickens infected by ingestion embryonated T. eggs. Under a microscope observation which were incubated petri dish containing 5 mL distilled water at 30 – 33◦C with regularly aerated, showed egg through various stages, including one cell, two cells, three four early morula, late blastula, gastrula, tadpole, pre-larva, larva. In addition, it took approximately 9 days for hatch infective develop - 33◦C. A total 50 randomly assigned 3 groups group I (10 chicks/group) served as control without inoculation; treatment II III (20 orally inoculated 500 or 1000 eggs, respectively. On 1, 3, 6, 15 post inoculation (dpi), chickens/control 4 chickens/treatment necropsied. results that percentage recovered varied from 14.00 33.93% 13.07 32.00% III, After dpi, significant differences about number found (P < 0.05). both groups, livers higher than lung tissue. For 6 small white foci on liver’s surface seen. Interstitial pneumonia, petechial hemorrhages, dark gray inflammatory nodules tissue atelectatic area observed. Histopathology examination revealed infiltrations leukocytes eosinophil scattered liver